Cht ceramic hydroxyapatite instruction manual

Bio-Scale Ceramic Hydroxyapatite, Type I Columns Instruction Manual Catalog Numbers , , , For Technical Service Call Your Local Bio-Rad Office or in the U.S. Call BIORAD (). CHT Ceramic Hydroxyapatite XT Media (CHT XT) is a calcium affinity cation exchange chromatography media. It is engineered with advanced technology, resulting in unique selectivity and robust, uniformly-sized beads with excellent pressure-flow properties. The chemical composition of CHT XT is (Ca 10 (PO 4) 6 (OH) 2) and it is a mixed-mode www.doorway.ruonal group: Ca 2+, PO 4, OH. of ceramic hydroxyapatite, CHT Types I and II and CHT XT. The temperature of the sintering step in part determines whether the ceramic hydroxyapatite is CHT Type I or Type II or CHT XT. CHT is a mixed-mode resin. Details of the binding mechanism are described in Chapter 3.

Hydroxyapatite Chromatography. Ca⁺⁺Pure-HA is a hydroxyapatite resin used in the chromatographic separation of www.doorway.ru applications for use of Ca⁺⁺Pure-HA include the purification of monoclonal and polyclonal antibodies, including IgG, IgA, IgM, antibody fragments, fusion- and phosphoproteins, and the separation of single-stranded and double-stranded DNA. Abstract. Past studies have shown that CHT Ceramic Hydroxyapatite Media can be used for virus purification. We purified influenza and dengue viruses on the newest member of the CHT family, CHT XT Media. We recovered over 75% of each virus in a single step of purification. In addition, 90% reduction of contaminating proteins and 60% removal of. pH range of separation with hydroxyapatite media. Materials and methods Hydroxyapatite media. Hydroxyapatite media was obtainedfromBio-RadLaboratories(Hercules,CA)as Macro-Prep ceramic hydroxapatite type II (CHT II), which is a spherical, macroporous form of crystalline calcium phosphate with particles 40lm in diameter (Catalogue No. ).

CHT Ceramic Hydroxyapatite XT Media (CHT XT) is a calcium affinity cation exchange chromatography media. It is engineered with advanced technology, resulting in unique selectivity and robust, uniformly-sized beads with excellent pressure-flow properties. The chemical composition of CHT XT is (Ca 10 (PO 4) 6 (OH) 2) and it is a mixed-mode media. CHT Ceramic Hydroxyapatite (Ca 5 (PO 4) 3 OH) 2 support is a leading purification medium for today's demanding downstream process industry. This mixed-mode support offers unique selectivities and often separates biomolecules that appear homogeneous using other chromatographic methods. The diverse binding capabilities of CHT media for host cell. CHT ceramic hydroxyapatite is a spherical, macroporous form of hydroxyapatite. It has been sintered at high temperatures to modify it from a crystalline to a ceramic form. The ceramic material overcomes many of the limitations of traditional crystalline hydroxyapatite that prevent its use in industrial-scale applications.